Strain Design

Engineering a microbial chassis to produce a target chemical. Carbon flux routes through aerobic TCA (high yield, expensive O₂) or overflow fermentation (cheap, lower yield). Both converge at product recovery.

Decision logic

Compare alternative production paths and see which route wins under shared yield, budget, and emissions constraints.

Decision question

Which metabolic route should we run if we care about yield, budget, and emissions at the same time?

Model output

Recommended routeAerobic until O₂ caps

Best achievable yield4.8 mmol/gDW/hr

Flip point+1 aeration step makes aerobic dominant

Active limits

Aeration capped
Media budget 88% used

Key insight

Aerobic wins on yield, overflow stays cheaper and more flexible, and the recommendation flips only when aeration expands.

Aerobic route leads on yield, but only while aeration can support it.

Overflow stays available as the lower-yield fallback when O₂ is constrained.

Expand aeration and the route choice becomes obvious.

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